In Vitro Cytotoxic Activity of the Ethanolic Leaf Extract of Myristica fragrans Houtt. On MCF-7 Breast Cancer Cells In Vitro Cytotoxic Activity of the Ethanolic Leaf Extract of Myristica fragrans Houtt. On MCF-7 Breast Cancer Cells
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Abstract
The high mortality rate due to breast cancer in women globally, including in Indonesia, has triggered the urgency of finding alternative therapies that are more affordable and have minimal side effects. This study aims to evaluate the cytotoxic effects of ethanolic leaf extract of nutmeg (Myristica fragrans Houtt) on the MCF-7 breast cancer cell line. The extraction process was carried out by maceration with 70% ethanol solvent, followed by cell viability testing using the PrestoBlue assay at various concentrations from 7.81 to 1000 µg/mL. In this experiment, cisplatin was applied as a positive control, while DMSO along with culture media acted as negative controls and untreated MCF-7 Cells. The test results showed that increasing the extract concentration caused a gradual decrease in MCF-7 cell viability, with the most obvious inhibitory effect seen in the morphology of cells exposed to concentrations of 250, 500 and 1000 µg/mL. The IC50 value obtained was 415.80 µg/mL, so its cytotoxic activity was categorized as moderate cytotoxic.
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References
Alshaya, D.S. and Awad, N.S. (2022) ‘Antiproliferative Effect of Clitoria ternatea Ethanolic Extract against Colorectal, Breast, and Medullary Thyroid Cancer Cell Lines’.
Arbiastutie, Y., Diba, F. and Masriani, M. (2022) ‘Cytotoxicity Activity of Several Medicinal Plants Grow in Mangrove Forest against Human’s Cervical (HELA), Breast (T47D), and Colorectal (WiDr) Cancer Cell Lines’, pp. 46–50. Available at: https://doi.org/10.4103/ijnpnd.ijnpnd.
Basir, A., Baja, S. and Lopulisa, C. (2018) ‘Biosciences and Plant Biology Analysis of Land Characteristics (Soil and Climate) for Nutmeg Suitability Evaluation (Myristica fragrans Houtt) in The Banda Naire Island of Central Maluku Districts’, 5(12), pp. 10–16.
Cragg, G.M. and Newman, D.J. (2005) ‘Plants as a source of anti-cancer agents’, Journal of Ethnopharmacology, 100(1–2), pp. 72–79. Available at: https://doi.org/10.1016/j.jep.2005.05.011.
Hanahan, D. (2022) ‘Hallmarks of Cancer: New Dimensions’, Cancer Discovery, 12(1), pp. 31–46. Available at: https://doi.org/10.1158/2159-8290.CD-21-1059.
Horwitz, K.B., Costlow, M.E. and McGuire, W.L. (1975) ‘MCF-7; a human breast cancer cell line with estrogen, androgen, progesterone, and glucocorticoid receptors’, Steroids, 26(6), pp. 785–795. Available at: https://doi.org/10.1016/0039-128X(75)90110-5.
International Agency for Research on Cancer (2020) ‘Global cancer observatory (GLOBOCAN)’. Available at: https://gco.iarc.fr/.
Komeda, S. (2019) ‘Different Effects of Cisplatin and Transplatin on the Higher-Order Structure of DNA and Gene Expression’, (Ii).
Le, T.T. et al. (2024) ‘Neolignans and Diarylnonanoid Derivatives with Anti-inflammatory Activity from’. Available at: https://doi.org/10.1021/acsomega.4c05649.
Lesmana, R. et al. (2021) ‘The Effect of Nutmeg Seed (M. fragrans) Extracts Induces Apoptosis in Melanoma Maligna Cells (B16-F10)’, 13(1). Available at: https://doi.org/10.18585/inabj.v13i1.1424.
Luzak, B. and Boncler, M. (2022) ‘Toxicology in Vitro An evaluation of a new high-sensitivity PrestoBlue assay for measuring cell viability and drug cytotoxicity using EA. hy926 endothelial cells’, 83(February). Available at: https://doi.org/10.1016/j.tiv.2022.105407.
Lüönd, F., Tiede, S. and Christofori, G. (2021) ‘Breast cancer as an example of tumour heterogeneity and tumour cell plasticity during malignant progression’, British Journal of Cancer [Preprint], (April). Available at: https://doi.org/10.1038/s41416-021-01328-7.
Nur, A. et al. (2024) ‘Efektivitas ekstrak daging buah pala (Myristica fragrans Houtt) terhadap histopatologi hati pada tikus hiperglikemia’.
Rajih, M.F. et al. (2023) ‘Aktivitas Antijamur Ekstrak Etanol Biji Pala (Myristica Fragrans Houtt .) Terhadap Malassezia furfur dan Tricophyton mentagrophytes’, 6(1), pp. 67–75.
Rampersad, S.N. (2012) ‘Multiple Applications of Alamar Blue as an Indicator of Metabolic Function and Cellular Health in Cell Viability Bioassays’, pp. 12347–12360. Available at: https://doi.org/10.3390/s120912347.
Saputri, A. et al. (2024) ‘Phytochemical Profiles, Antioxidant, And Anticancer Activities From Leaves And Seeds Extract Of Myristica fragrans’, 9(2), pp. 182–195.
Vieira-da-Silva, B. and Castanho, M.A.R.B. (2023) ‘Resazurin Reduction-Based Assays Revisited: Guidelines for Accurate Reporting of Relative Differences on Metabolic Status’, Molecules, 28(5), 2283. Available at: https://doi.org/10.3390/molecules28052283.
Wang, W. et al. (2025) ‘Targeting MDM2, RAS, and PCNA for cancer targeted therapy : pan-cancer approaches vs. cancer specific strategies’, (October). Available at: https://doi.org/10.3389/fphar.2025.1663766.
World Health Organization (2025) ‘World Health Organization’. Available at: https://www.who.int/.
Zafar, A. et al. (2025) ‘Advancements and limitations in traditional anti-cancer therapies : a comprehensive review of surgery, chemotherapy, radiation therapy, and hormonal therapy’, Discover Oncology [Preprint]. Available at: https://doi.org/10.1007/s12672-025-02198-8.